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SINGLE CELL OMICS

Single cell genomics allows for the high resolution assessment of hundreds to tens of thousands of individual cells, unmasking the heterogeneity of a cell population and characterizing cell types and states on a cell-by-cell basis.  

SINGLE CELL 10x GENOMICS

3' scRNA-SEQ

  • Optional: with cell surface protein expression and/or sample multiplexing

  • Includes capture 500 - 10,000 cells

  • Library Preparation, QC Metrics

  • Optional: Processing of cryopreserved cells

  • Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

3' scRNA-SEQ + CRISPR SCREENING

  • Includes capture 500 - 10,000 cells

  • Library Preparation, QC Metrics

  • Optional: Processing of cryopreserved cells

  • Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scATAC-SEQ

  • Includes capture 500 - 10,000 nuclei (nuclei isolation from 100,000 - 1,000,000

  • Library Preparation, QC Metrics

  • Optional: Processing of cryopreserved cells

  • Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scCNV

  • Includes capture 250 - 5,000 cells

  • Library Preparation, QC Metrics

  • Optional: Processing of cryopreserved cells

  • Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scV(D)J, B or T CELL ENRICHMENT

  • Includes capture 500 10,000 cells

  • Library Preparation, QC Metrics

  • Optional: Processing of cryopreserved cells

  • Optional: Dead cell removal

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  • Optional: with 5' scRNA-seq

  • Optional: with cell surface protein expression and/or sample multiplexing

Deliverables include outputs from cell ranger, FASTQ files

10x GENOMICS SEQUENCING ONLY

10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)

  • 91 bp transcript read

  • 400M

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)

  • 2 x 70 bp

  • 2 x 130M, 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)

  • 2 x 50 bp

  • 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scCNV LIBRARIES (NOVASEQ)

  • Option 1

    • 2 x 100 bp​

    • 2 x 1.6B reads

  • Option 2

    • 2 x 150 bp​

    • 2 x 800M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (MISEQ)

  • 2 x 150 bp

  • 2 x 15M reads​

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)

  • 2 x 150 bp

  • 2 x 400M reads​

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)

  • 91 bp transcript read

  • 400M reads​

Deliverables include outputs from CellRanger, FASTQ files

PLATE-BASED WORKFLOWS

FULL TRANSCRIPTOME scRNA-SEQ

  • Plate Based

  • Cell lysis, RT, cDNA amplification

  • Library Preparation, QC

  • cDNA QC

Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)

BD BIOSCIENCES RHAPSODY SINGLE CELL ANALYSIS SYSTEM

LIBRARY PREPARATION AND QC

  • Predesigned panels scRNA-seq

    • Plate Based

    • Cell lysis, RT, cDNA amplification, QC

      • Onco-BC panel

      • T-Cell panel

      • 1R (human mouse) panel

Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)

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