SINGLE CELL OMICS

3' scRNA-SEQ

• Optional: with cell surface protein expression and/or sample multiplexing

• Includes capture 500 - 10,000 cells

• Library Preparation, QC Metrics

• Optional: Processing of cryopreserved cells

• Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

3' scRNA-SEQ + CRISPR SCREENING

• Includes capture 500 - 10,000 cells

• Library Preparation, QC Metrics

• Optional: Processing of cryopreserved cells

• Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scATAC-SEQ

• Includes capture 500 - 10,000 nuclei (nuclei isolation from 100,000 - 1,000,000

• Library Preparation, QC Metrics

• Optional: Processing of cryopreserved cells

• Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scCNV

• Includes capture 250 - 5,000 cells

• Library Preparation, QC Metrics

• Optional: Processing of cryopreserved cells

• Optional: Dead cell removal

Deliverables include outputs from cell ranger, FASTQ files

scV(D)J, B or T CELL ENRICHMENT

• Includes capture 500 10,000 cells

• Library Preparation, QC Metrics

• Optional: Processing of cryopreserved cells

• Optional: Dead cell removal

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• Optional: with 5' scRNA-seq

• Optional: with cell surface protein expression and/or sample multiplexing

Deliverables include outputs from cell ranger, FASTQ files

10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)

• 91 bp transcript read

• 400M

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)

• 2 x 70 bp

• 2 x 130M, 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)

• 2 x 50 bp

• 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scCNV LIBRARIES (NOVASEQ)

• Option 1

  • 2 x 100 bp​

  • 2 x 1.6B reads

• Option 2

  • 2 x 150 bp​

  • 2 x 800M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (MISEQ)

• 2 x 150 bp

• 2 x 15M reads​

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)

• 2 x 150 bp

• 2 x 400M reads​

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)

• 91 bp transcript read

• 400M reads​

Deliverables include outputs from CellRanger, FASTQ files

FULL TRANSCRIPTOME scRNA-SEQ

• Plate Based

• Cell lysis, RT, cDNA amplification

• Library Preparation, QC

• cDNA QC

Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)

LIBRARY PREPARATION AND QC

• Predesigned panels scRNA-seq

  • Plate Based

  • Cell lysis, RT, cDNA amplification, QC

    • Onco-BC panel

    • T-Cell panel

    • 1R (human mouse) panel

Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)