SINGLE CELL OMICS
Single cell genomics allows for the high resolution assessment of hundreds to tens of thousands of individual cells, unmasking the heterogeneity of a cell population and characterizing cell types and states on a cell-by-cell basis.
SINGLE CELL 10x GENOMICS
3' scRNA-SEQ
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Optional: with cell surface protein expression and/or sample multiplexing
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Includes capture 500 - 10,000 cells
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Library Preparation, QC Metrics
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Optional: Processing of cryopreserved cells
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Optional: Dead cell removal
Deliverables include outputs from cell ranger, FASTQ files
3' scRNA-SEQ + CRISPR SCREENING
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Includes capture 500 - 10,000 cells
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Library Preparation, QC Metrics
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Optional: Processing of cryopreserved cells
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Optional: Dead cell removal
Deliverables include outputs from cell ranger, FASTQ files
scATAC-SEQ
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Includes capture 500 - 10,000 nuclei (nuclei isolation from 100,000 - 1,000,000
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Library Preparation, QC Metrics
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Optional: Processing of cryopreserved cells
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Optional: Dead cell removal
Deliverables include outputs from cell ranger, FASTQ files
scCNV
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Includes capture 250 - 5,000 cells
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Library Preparation, QC Metrics
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Optional: Processing of cryopreserved cells
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Optional: Dead cell removal
Deliverables include outputs from cell ranger, FASTQ files
scV(D)J, B or T CELL ENRICHMENT
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Includes capture 500 10,000 cells
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Library Preparation, QC Metrics
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Optional: Processing of cryopreserved cells
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Optional: Dead cell removal
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Optional: with 5' scRNA-seq
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Optional: with cell surface protein expression and/or sample multiplexing
Deliverables include outputs from cell ranger, FASTQ files
10x GENOMICS SEQUENCING ONLY
10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)
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91 bp transcript read
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400M
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)
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2 x 70 bp
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2 x 130M, 2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)
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2 x 50 bp
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2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scCNV LIBRARIES (NOVASEQ)
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Option 1
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2 x 100 bp
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2 x 1.6B reads
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Option 2
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2 x 150 bp
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2 x 800M reads
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Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scV(D)J LIBRARIES (MISEQ)
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2 x 150 bp
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2 x 15M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)
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2 x 150 bp
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2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)
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91 bp transcript read
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400M reads
Deliverables include outputs from CellRanger, FASTQ files
PLATE-BASED WORKFLOWS
FULL TRANSCRIPTOME scRNA-SEQ
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Plate Based
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Cell lysis, RT, cDNA amplification
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Library Preparation, QC
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cDNA QC
Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)
BD BIOSCIENCES RHAPSODY SINGLE CELL ANALYSIS SYSTEM
LIBRARY PREPARATION AND QC
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Predesigned panels scRNA-seq
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Plate Based
- Cell lysis, RT, cDNA amplification, QC
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Onco-BC panel
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T-Cell panel
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1R (human mouse) panel
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Deliverables include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files)