SEQUENCING

This service includes fragment analysis, Qubit or qPCR quantification, and sequencing of pre-pooled, multiplexed libraries. Full runs only (no flow cell or lane sharing). For non-standard sequencing runs, consult Illumina technical support at techsupport@illumina.com and provide finalized sequencing parameters to the AGCT Core.

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Specific Submission Requirements

SEQUENCING PLATFORM: NEXTSEQ 5000 SYSTEM

Sequencing Capabilities Include

1 x 75 bp, High (30 Gb)

2 x 75 bp, High (60 Gb) or Mid (19.5 Gb)

2 x 150 bp, High (120 Gb) or Mid (39 Gb)

Delivarable include FASTQ files

SEQUENCING PLATFORM: NOVASEQ 6000 SYSTEM

Sequencing Capabilities Include

2 x 50 bp
- SP (65-80 Gb)
- S1 (134-167 Gb)
- S2 (333-417 Gb)

2 x 100 bp
- S1 (266-333 Gb)
- S2 (667-833 Gb)
- S4 (1600-2000 Gb)

2 x 150 bp
- SP (200-250 Gb)
- S1 (400-500 Gb)
- S2 (1000-1250 Gb)
- S4 (2400-3000 Gb)

2 x 250 bp
- SP (325-400 Gb)

Delivarable include FASTQ files

SEQUENCING PLATFORM: MISEQ, REAGENT KIT V2

Sequencing Capabilities Include

2 x 25 bp
- 0.75 - 0.85 Gb

2 x 150 bp
- Nano (0.3 Gb)
- Micro (1.2 Gb)
- 4.5 - 5.1 Gb

2 x 250 bp
- Nano (0.5 Gb)
- 8.5 Gb

Delivarable include FASTQ files

SEQUENCING PLATFORM: MISEQ, REAGENT KIT V3

Sequencing Capabilities Include

2 x 75 bp
- 3.3 - 3.8 Gb

2 x 250 bp
- 7.5- 8.5 Gb

2 x 300 bp
- 13.2 - 15 Gb

Delivarable include FASTQ files

10x GENOMICS SEQUENCING ONLY

10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)

91 bp transcript read
400M

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)

2 x 70 bp
2 x 130M, 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)

2 x 50 bp
2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scCNV LIBRARIES (NOVASEQ)

Option 1
- 2 x 100 bp
- 2 x 1.6B reads

Option 2
- 2 x 150 bp
- 2 x 800M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (MISEQ)

2 x 150 bp
2 x 15M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)

2 x 150 bp
2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)

91 bp transcript read
400M reads

Deliverables include outputs from CellRanger, FASTQ files