SEQUENCING

This service includes fragment analysis, Qubit or qPCR quantification, and sequencing of pre-pooled, multiplexed libraries.  Full runs only (no flow cell or lane sharing).  For non-standard sequencing runs, consult Illumina technical support at techsupport@illumina.com and provide finalized sequencing parameters to the AGCT Core. 

SEQUENCING PLATFORM: NEXTSEQ 5000 SYSTEM

Sequencing Capabilities Includ​e

  • 1 x 75 bp, High (30 Gb)

  • 2 x 75 bp, High (60 Gb) or Mid (19.5 Gb)

  • 2 x 150 bp, High (120 Gb) or Mid (39 Gb)

Delivarable include FASTQ files​

SEQUENCING PLATFORM: NOVASEQ 6000 SYSTEM

Sequencing Capabilities Include

  • 2 x 50 bp

    • SP (65-80 Gb)

    • S1 (134-167 Gb)

    • S2 (333-417 Gb)​​

  • 2 x 100 bp​​

    • S1 (266-333 Gb)

    • S2 (667-833 Gb)​​

    • S4 (1600-2000 Gb)

  • 2 x 150 bp​​

    • SP (200-250 Gb)

    • S1 (400-500 Gb)​​

    • S2 (1000-1250 Gb)

    • S4 (2400-3000 Gb)

  • 2 x 250 bp​​

    • SP (325-400 Gb)

Delivarable include FASTQ files​

SEQUENCING PLATFORM: MISEQ, REAGENT KIT V2

Sequencing Capabilities Include

  • 2 x 25 bp

    • 0.75 - 0.85 Gb​

  • 2 x 150 bp

    • ​Nano (0.3 Gb)

    • Micro (1.2 Gb)

    • 4.5 - 5.1 Gb

  • 2 x 250 bp

    • ​Nano (0.5 Gb)

    • 8.5 Gb

Delivarable include FASTQ files​

SEQUENCING PLATFORM: MISEQ, REAGENT KIT V3

Sequencing Capabilities Include

  • 2 x 75 bp

    • 3.3 - 3.8 Gb​

  • 2 x 250 bp

    • 7.5- 8.5 Gb​

  • 2 x 300 bp

    • 13.2 - 15 Gb​

Delivarable include FASTQ files​

10x GENOMICS SEQUENCING ONLY

10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)

  • 91 bp transcript read

  • 400M

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)

  • 2 x 70 bp

  • 2 x 130M, 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)

  • 2 x 50 bp

  • 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scCNV LIBRARIES (NOVASEQ)

  • Option 1

    • 2 x 100 bp​

    • 2 x 1.6B reads

  • Option 2

    • 2 x 150 bp​

    • 2 x 800M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (MISEQ)

  • 2 x 150 bp

  • 2 x 15M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)

  • 2 x 150 bp

  • 2 x 400M reads

Deliverables include outputs from CellRanger, FASTQ files

10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)

  • 91 bp transcript read

  • 400M reads

Deliverables include outputs from CellRanger, FASTQ files