SEQUENCING
This service includes fragment analysis, Qubit or qPCR quantification, and sequencing of pre-pooled, multiplexed libraries. Full runs only (no flow cell or lane sharing). For non-standard sequencing runs, consult Illumina technical support at techsupport@illumina.com and provide finalized sequencing parameters to the AGCT Core.
Sequencing Capabilities Include
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1 x 75 bp, High (30 Gb)
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2 x 75 bp, High (60 Gb) or Mid (19.5 Gb)
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2 x 150 bp, High (120 Gb) or Mid (39 Gb)
Delivarable include FASTQ files
Sequencing Capabilities Include
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2 x 50 bp
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SP (65-80 Gb)
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S1 (134-167 Gb)
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S2 (333-417 Gb)
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2 x 100 bp
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S1 (266-333 Gb)
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S2 (667-833 Gb)
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S4 (1600-2000 Gb)
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2 x 150 bp
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SP (200-250 Gb)
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S1 (400-500 Gb)
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S2 (1000-1250 Gb)
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S4 (2400-3000 Gb)
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2 x 250 bp
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SP (325-400 Gb)
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Delivarable include FASTQ files
Sequencing Capabilities Include
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2 x 25 bp
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0.75 - 0.85 Gb
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2 x 150 bp
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Nano (0.3 Gb)
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Micro (1.2 Gb)
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4.5 - 5.1 Gb
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2 x 250 bp
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Nano (0.5 Gb)
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8.5 Gb
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Delivarable include FASTQ files
Sequencing Capabilities Include
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2 x 75 bp
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3.3 - 3.8 Gb
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2 x 250 bp
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7.5- 8.5 Gb
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2 x 300 bp
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13.2 - 15 Gb
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Delivarable include FASTQ files
10x GENOMICS SEQUENCING ONLY
10x GENOMICS 3' scRNA-SEQ LIBRARIES (NOVASEQ)
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91 bp transcript read
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400M
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scATAC-SEQ LIBRARIES (NEXTSEQ)
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2 x 70 bp
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2 x 130M, 2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scATAC-SEQ LIBRARIES (NOVASEQ)
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2 x 50 bp
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2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scCNV LIBRARIES (NOVASEQ)
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Option 1
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2 x 100 bp
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2 x 1.6B reads
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Option 2
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2 x 150 bp
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2 x 800M reads
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Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scV(D)J LIBRARIES (MISEQ)
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2 x 150 bp
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2 x 15M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS scV(D)J LIBRARIES (NOVASEQ)
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2 x 150 bp
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2 x 400M reads
Deliverables include outputs from CellRanger, FASTQ files
10x GENOMICS 5' scRNA-SEQ LIBRARIES (NOVASEQ)
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91 bp transcript read
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400M reads
Deliverables include outputs from CellRanger, FASTQ files