TRANSCRIPTOMICS

RNA-Seq is a highly sensitive and accurate tool for investigating and discovering the whole transcriptome.  It allows for differential gene expression profiling, identification of known and novel transcript isoforms, alternative splicing events, gene fusions, and post-transcriptional modifications.  We offer services for total RNA-Seq, mRNA-Seq, and small RNA-Seq, and for high and low quality samples.  All standard services include RNA fluorometric quantification and integrity analysis, library preparation, library fluorometric quantification and fragment analysis, and Illumina single-end sequencing. The service also includes demultiplexing, alignment, expression quantification and quality control. 

mRNA-SEQ

  • Option 1 (NextSeq)

    • 1 x 75 bp

    •  30M (gene expression)

  • Option 2 (NovaSeq)

    • 2 x 50 bp

    • 2 x 30M 

Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files

ULTRA LOW-INPUT mRNA-SEQ, RNA INPUT

  • Option 1 (NextSeq)

    • 1 x 75 bp

    • 30M

  • Option 2 (NovaSeq)

    • 2 x 50 bp

    • 2 x 30M 

Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files

ULTRA LOW-INPUT mRNA-SEQ, WHOLE CELL INPUT

  • Option 1 (NextSeq)

    • 1 x 75 bp

    • 30M

  • Option 2 (NovaSeq)

    • 2 x 50 bp

    • 2 x 30M 

Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files

3' mRNA-SEQ FOR DEGRADED OR FFPE RNA (UMI QUANTIFICATION)

  • Options: NextSeq Mid Output, NextSeq High Output, NovaSeq

    • 1x75bp

    • 10M (gene sequencing only)

Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files

TOTAL RNA-SEQ

  • Option 1 (NextSeq)

    • 1 x 75 bp

    • 50M 

  • Option 2 (NovaSeq)

    • 2 x 50 bp

    • 2 x 50M

Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files

miRNA-SEQ (UMI QUANTIFICATION)

  • Options: NextSeq Mid Output, NextSeq High Output, NovaSeq

    • 1 x 75 bp

    • 10M 

Deliverable include raw/normalized expression analysis, excel QC report, FASTQ files

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