TRANSCRIPTOMICS
RNA-Seq is a highly sensitive and accurate tool for investigating and discovering the whole transcriptome. It allows for differential gene expression profiling, identification of known and novel transcript isoforms, alternative splicing events, gene fusions, and post-transcriptional modifications. We offer services for total RNA-Seq, mRNA-Seq, and small RNA-Seq, and for high and low quality samples. All standard services include RNA fluorometric quantification and integrity analysis, library preparation, library fluorometric quantification and fragment analysis, and Illumina single-end sequencing. The service also includes demultiplexing, alignment, expression quantification and quality control.
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Option 1 (NextSeq)
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1 x 75 bp
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30M (gene expression)
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Option 2 (NovaSeq)
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2 x 50 bp
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2 x 30M
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Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files
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Option 1 (NextSeq)
-
1 x 75 bp
-
30M
-
-
Option 2 (NovaSeq)
-
2 x 50 bp
-
2 x 30M
-
Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files
-
Option 1 (NextSeq)
-
1 x 75 bp
-
30M
-
-
Option 2 (NovaSeq)
-
2 x 50 bp
-
2 x 30M
-
Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files
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Options: NextSeq Mid Output, NextSeq High Output, NovaSeq
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1x75bp
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10M (gene sequencing only)
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Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files
-
Option 1 (NextSeq)
-
1 x 75 bp
-
50M
-
-
Option 2 (NovaSeq)
-
2 x 50 bp
-
2 x 50M
-
Deliverable include gene count matrix (TPM/COUNT), QC summary table, MultiQC report, FASTQ files
-
Options: NextSeq Mid Output, NextSeq High Output, NovaSeq
-
1 x 75 bp
-
10M
-
Deliverable include raw/normalized expression analysis, excel QC report, FASTQ files