EPIGENOMICS

The epigenome is the complete description of all the chemical modifications to DNA and histone proteins that regulate the expression of genes within the genome. These modifications occur as a natural process of development and tissue differentiation, and can be altered in response to environmental exposures or disease. We offer three methods for epigenetic analysis; ATAC-Seq for examining the accessibility of chromatin, ChIP-Seq for profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins, and WGBS for interrogating the methylation status of the genome. We also offer single cell ATAC-Seq.

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Specific Submission Requirements

ATAC-SEQ

Option 1
- Nucleosome Positioning
- Sequencing: 2 x 75 bp; 2 x 40M reads

Option 2
- TF Profiling
- Sequencing: 2 x 50 bp; 2 x 120M reads

Includes transposition, library preparation, QC Metrics

Deliverables include excel peak calling table, QC report, FASTQ files

ChIP-SEQ (HISTONE MODIFICATION, H3K27ac)

Sequencing: 1 X 75 bp, 25M reads

Includes ChIP pulldown, qPCR, library preparation, QC metrics

Deliverables include excel peak calling table, QC report, FASTQ files

ChIP-SEQ (LIBRARY PREPARATION AND SEQUENCING ONLY)

Option 1
- Histone Profiling
- Sequencing: 1 X 75 bp, 50M reads

Option 2
- TF Profiling
- Sequencing: 1 x 75 bp, 25M reads

Includes Library Preparation, QC metrics

Deliverables include excel peak calling table, QC report, FASTQ files

WHOLE GENOME BISULFITE SEQUENCING (WGBS)

Sequencing: 2 x 150 bp ; 30x coverage

Includes Library Preparation, QC Metrics

Deliverables include bedgraph with CpG sites, bedgraph with all C, summary QC table, MultiQC report, FASTQ files