top of page

EPIGENOMICS

The epigenome is the complete description of all the chemical modifications to DNA and histone proteins that regulate the expression of genes within the genome.  These modifications occur as a natural process of development and tissue differentiation, and can be altered in response to environmental exposures or disease.  We offer three methods for epigenetic analysis; ATAC-Seq for examining the accessibility of chromatin, ChIP-Seq for profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins, and WGBS for interrogating the methylation status of the genome.  We also offer single cell ATAC-Seq.

ATAC-SEQ

  • Option 1

    • Nucleosome Positioning

    • Sequencing: 2 x 75 bp; 2 x 40M reads

  • Option 2

    • TF Profiling

    • Sequencing: 2 x 50 bp; 2 x 120M reads

Includes transposition, library preparation, QC Metrics​

Deliverables include excel peak calling table, QC report, FASTQ files

ChIP-SEQ (HISTONE MODIFICATION, H3K27ac)

  • Sequencing: 1 X 75 bp, 25M reads

Includes ChIP pulldown, qPCR, library preparation, QC metrics​

Deliverables include excel peak calling table, QC report, FASTQ files

ChIP-SEQ (LIBRARY PREPARATION AND SEQUENCING ONLY)

  • Option 1

    • Histone Profiling​

    • Sequencing: 1 X 75 bp, 50M reads

  • Option 2

    • TF Profiling

    • Sequencing: 1 x 75 bp, 25M reads

Includes Library Preparation, QC metrics

Deliverables include excel peak calling table, QC report, FASTQ files

WHOLE GENOME BISULFITE SEQUENCING (WGBS)

  • Sequencing: 2 x 150 bp ; 30x coverage

Includes Library Preparation, QC Metrics

Deliverables include bedgraph with CpG sites, bedgraph with all C, summary QC table, MultiQC report, FASTQ files

bottom of page