EPIGENOMICS
The epigenome is the complete description of all the chemical modifications to DNA and histone proteins that regulate the expression of genes within the genome. These modifications occur as a natural process of development and tissue differentiation, and can be altered in response to environmental exposures or disease. We offer three methods for epigenetic analysis; ATAC-Seq for examining the accessibility of chromatin, ChIP-Seq for profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins, and WGBS for interrogating the methylation status of the genome. We also offer single cell ATAC-Seq.
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Option 1
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Nucleosome Positioning
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Sequencing: 2 x 75 bp; 2 x 40M reads
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Option 2
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TF Profiling
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Sequencing: 2 x 50 bp; 2 x 120M reads
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Includes transposition, library preparation, QC Metrics
Deliverables include excel peak calling table, QC report, FASTQ files
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Sequencing: 1 X 75 bp, 25M reads
Includes ChIP pulldown, qPCR, library preparation, QC metrics
Deliverables include excel peak calling table, QC report, FASTQ files
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Option 1
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Histone Profiling
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Sequencing: 1 X 75 bp, 50M reads
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Option 2
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TF Profiling
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Sequencing: 1 x 75 bp, 25M reads
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Includes Library Preparation, QC metrics
Deliverables include excel peak calling table, QC report, FASTQ files
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Sequencing: 2 x 150 bp ; 30x coverage
Includes Library Preparation, QC Metrics
Deliverables include bedgraph with CpG sites, bedgraph with all C, summary QC table, MultiQC report, FASTQ files