Single Cell DNA-Seq

Single cell genomics allows for the high resolution assessment of hundreds to tens of thousands of individual cells, unmasking the heterogeneity of a cell population and characterizing cell types and states on a cell-by-cell basis.  Our suite of single cell technologies includes droplet-based (10x Genomics Chromium), microwell-based (BD Biosciences Rhapsody), and plate-based (via FACS) methods.  We offer many single cell transcriptomics applications, including gene expression profiling, gene & cell surface protein profiling, immune profiling, immune & cell surface protein profiling, immune profiling & antigen specificity, and gene expression CRISPR screening.  Combine single cell transcriptomics with single cell chromatin accessibility for a multi-omics approach.  Or examine copy number variation of up to 40,000 cells in parallel.  In addition to the standard services listed below, we are actively increasing our repertoire of single cell genomics offerings in this highly evolving field.  See our Research & Development page to view our latest progress.

Mission Bio Tapestri System

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The Mission Bio Tapestriplatform is the first and only single cell multi-omics platform capable of detecting SNVs, CNVs, and protein changes simultaneously from the same cell. A two-step workflow first encapsulates a single cell together with protease, resulting in the release of the cell’s genomic DNA. Next, the cell lysate is encapsulated together with barcoding beads and reagent mix. Target amplification is achieved within the droplet, during which each amplicon is tagged with a cell barcode. The droplets are then broken to combine the amplified DNA into a single pool. Library construction is performed in bulk to add sample indices and sequencing primers, allowing for sequencing on the Illumina platform. The system is compatible with either fresh or frozen cells or tissue. An input of 100,000 cells/nuclei yields a single cell/nuclei capture of 5,000-10,000 cells/nuclei. The Tapestri platform can be used to detect rare subclones (down to 0.1%), identify true mutation co-occurance in clonal populations, resolve zygosity, andreconstruct phylogenetic lineages. Pre-designed targeted panels for hematologic and solid tumors available. Custom panels can be designed using the Tapestri Designer.