Sequencing
The sequencing only option is for investigators who want to prepare their own libraries, then use the Genomic Core's stand-alone sequencing service. This service includes fragment analysis, Qubit or qPCR quantification, and sequencing of pre-pooled, multiplexed libraries. Full runs only (no flow cell or lane sharing). For non-standard sequencing runs, consult Illumina technical support at techsupport@illumina.com and provide finalized sequencing parameters to the AGCT Core.
NovaSeq 6000
The NovaSeq 6000 system offers flexibility for virtually any sequencing method, genome, and scale. Patterned flow cell technology generates an unprecedented level of throughput for a broad range of sequencing applications. Four flow cells are available, as well as the NovaSeq XP workflow that allows for the loading of individual lanes with different library types. Up to 3000 Gb output, 10 B reads, and 2x250 bp read length. A default of 300 pM will be used if a loading concentration is not specified or if the AGCT Core has no prior experience with the library type. Sample requirements for standard services can be found here.
NextSeq 500
The NextSeq 500 system delivers the power and flexibility to carry out applications such as whole genome, exome, transcriptome, and methylation sequencing, among others. Two flow cell configurations allow for tunable outputs. Up to 120 Gb output, 400 M reads, and 2x150 bp read length. A default of 1.8 pM for high output flow cells and 1.5 pM for mid output flow cells will be used if a loading concentration is not specified or if the Genomics Core has no prior experience with the library type. Sample requirements for standard services can be found here.
MiSEQ
The MiSeq system offers the first end-to-end sequencing solution, integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. The MiSeq is best suited for whole genome sequencing of smaller model organism genomes, medium to small targeted panels, amplicon sequencing, 16S metagenomics, quality control of libraries, and more. Flow cells are available in 3 sizes. Up to 15 Gb output, 25 M reads, and 2x300 bp read length. A default of 12 pM for V2 kits and 20 pM V3 kits will be used if a loading concentration is not specified or if the Genomics Core has no prior experience with the library type. Sample requirements for standard services can be found here.
