The AGCT Core generates genomic, transcriptomic, epigenomic & metagenomic information for basic and clinical research. We make complex technologies and methodologies available and interpretable to the research and clinical community.
As a Certified Service Provider, we specialize in 10x Genomics Single Cell Sequencing and Spatial Gene Expression Assays.
BFG/AGCT Core Seminar Series
Speaker(s): Anja Bastian, PhD
Title(s): Getting Started with Single Cell Gene Expression Data Analysis using 10x Genomics Cloud Analysis and Loupe Browser
10X Genomics and Illumina
10x Genomics and Illumina jointly bring you a webinar by Dr. Jasmine Plummer from Cedars-Sinai Medical Center. Her webinar will focus on the combination of 10x technologies, single cell RNAseq and Visium with Illumina sequencing to reveal spatial resolution of the developing human fetal cortex.
Cedars-Sinai Medical Center's AGCT Core is a 10x Genomics Certified Service Provider.
The Singulator is a benchtop hands-off dissociator that provides both whole-cell and nuclei tissue dissociations. The S100 model comes with a chilling chamber for cold dissociation to decrease transcriptional stress response and dissociation bias for nuclei isolation. Protocols are quick and easy to use, with the ability to make small changes to suit the tissue of interest. Cells isolation take anywhere from 20-60min while nuclei only take 7min to process. The singulator is a new addition to our Sample Preparation workflow with high reproducibility and viability.
Latest Papers + Studies
Wenjuan Zhang, PhD; Brian D. Davis, BSc; Stephanie S. Chen, BSc; Jorge M. Sincuir Martinez, BS; Jasmine T. Plummer, PhD; Eric Vail, MD. Emergence of a Novel SARS-CoV-2 Variant in Southern California. JAMA. January 2021 PDF
Wenjuan Zhang, PhD; John Paul Govindavari, DO; Brian D. Davis, BSc; Stephanie S. Chen, BSc; Jong Taek Kim, MD; Jianbo Song, PhD; Jean Lopategui, MD; Jasmine T. Plummer, PhD; Eric Vail, MD. Analysis of Genomic Characteristics and Transmission Routes of Patients With Confirmed SARS-CoV-2 in Southern California During the Early Stage of the US COVID-19 Pandemic. JAMA Netw Open. 2020 PDF
Noushmehr H, Monteiro A, Pharoah P, Pasaniuc B, Gayther SA. A transcriptome-wide association study of high-grade serous epithelial ovarian cancer identifies new susceptibility genes and splice variants. Nature Genetics. May 2019 PDF
Rajamani U, Gross AR, Hjelm BE, Sequeira A, Vawter MP, Tang J, Gangalapudi V, Wang Y, Andres AM, Gottlieb RA, Sareen D.Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell Stem Cell. 2018 PDF
Skalski JH, Limon-Tello JJ, Sharma P, Gargus MD, Ngyuen C, Tang J, Coelho AL, Hogaboam CM, Crother TR, Underhill DM. Expansion of commensal fungus Wallemia mellicola in the gastrointestinal mycobiota enhances the severity of allergic airway disease in mice. PLOS Pathogens. 2018 PDF
Ljubimova JY, Braubach O, Patil R, Chiechi A, Tang J, Galstyan A, Shatalova ES, Kleinman MT, Black KL, Holler E. Coarse particulate matter (PM2.5-10) in Los Angeles Basin air induces expression of inflammation and cancer biomarkers in rat brains. Sci Rep. 2018 PDF
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